Membrane Systems Biology

Group leader: Kenji Maeda

We explore architecture and functionality of cancer cells with focus on membrane lipids. We elucidate how cancerous transformation alters the usage and needs for a variety of lipids by coupling systematic approaches of organelle purification with quantitative mass spectrometry-based lipidomics. Through the mapping of cellular distribution and metabolism for hundreds of lipids, we aim to identify key lipid metabolic pathways with crucial importance for cancer pathophysiology as new therapeutic targets.

Kenji Maeda

Kenji Maeda

Kenji Maeda has done his PhD at the Technical University of Denmark 2007, whereafter he became a postdoctoral fellow at EMBL Heidelberg 2008-2014.

Recipient of Sapere Aude DFF-Starting Grant 2016.

ORCID:

Kenji Maeda

0000-0002-9080-5691

Selected publications:

Nielsen, I. Ø., Maeda, K., Bilgin, M.
Global monitoring of the mammalian lipidome by quantitative shotgun lipidomics.
Methods Mol.Biol. 2017: 1609, 123-139

Bilgin, M., Nylandsted, J., Jäättelä, M., Maeda, K.
Quantitative profiling of lysosomal lipidome by shotgun lipidomics.
Methods Mol.Biol. 2017: 1594, 19-34

Boye, T. L., Maeda, K., Pezeshkian, W., Sønder, S. L., Häger, S. C., Gerke, V., Simonsen, A. C., Nylandsted, J.
Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair.
Nat.Commun. 2017: 8(1), 1623

Vonkova, I., Saliba, A. E., Deghou, S., Anand, K., Ceschia, S., Doerks, T., Galih, A., Kugler, K. G., Maeda, K., Rybin, V., van, Noort, V, Ellenberg, J., Bork, P., Gavin, A. C.
Lipid cooperativity as a general membrane-recruitment principle for PH domains.
Cell Rep. 2015: 12(9), 1519-1530

Maeda, K., Poletto, M., Chiapparino, A., Gavin, A. C.
A generic protocol for the purification and characterization of water-soluble complexes of affinity-tagged proteins and lipids.
Nat.Protoc. 2014: 9(9), 2256-2266

Saliba, A. E., Vonkova, I., Ceschia, S., Findlay, G. M., Maeda, K., Tischer, C., Deghou, S., van Noort, V., Bork, P., Pawson, T., Ellenberg, J., Gavin, A. C.
A quantitative liposome microarray to systematically characterize protein-lipid interactions.
Nat.Methods 2014: 11(1), 47-50

Maeda, K., Anand, K., Chiapparino, A., Kumar, A., Poletto, M., Kaksonen, M., Gavin, A. C.
Interactome map uncovers phosphatidylserine transport by oxysterol-binding proteins.
Nature 2013: 501(7466), 257-261