Bioimaging

The Bioimaging Core Facility is a technology platform that offers access to a wide range of light microscopes. We host equipment and provide training, expertise and analysis support. The main purpose of the core facility is to improve and maintain the quality of light microscopy for all researchers at the DCRC and keep our research technologically up-to-date.

Currently we have ten bookable microscopes that cover technologies such as:

• Confocal microscopy (Zeiss LSM700 and LSM800 with airyscan)
• High-throughput screening (2x Olympus ScanR, Molecular Devices ImageXpress Microconfocal and ImageXpresss Pico)
• Microirradiation (Zeiss PALM microirradiation and Perkin Elmer Ultraview Vox with microirradiation by Rapp OptoElectronic)
• In early 2021 we will install a Zeiss Elyra 7 super-resolution microscope
• Most of the microscopes are equipped with incubation chambers for live-cell imaging experiments

In addition we run three powerful analysis work stations with specialized software for image processing and analysis. We have licenses for:

• Olympus ScanR analysis including 3D deconvolution, Kinetics and Artificial Intelligence
• Molecular Devices MetaXpress
• Quorum Technologies Volocity
• SVI Huygens deconvolution and 3D movie rendering
• Tibco Spotfire for data visualization
• Zeiss Zen

We also run open-source image processing/analysis softwares packages, such as ImageJ (FIJI), Python, R, Cell Profiler etc.